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Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli

To examine the effects of overexpression of trigger factor (TF) on recombinant proteins produced in Escherichia coli, we constructed plasmids that permitted controlled expression of TF alone or together with the GroEL-GroES chaperones. The following three proteins that are prone to aggregation were...

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Bibliografski detalji
Glavni autori: Nishihara, Kazuyo, Kanemori, Masaaki, Yanagi, Hideki, Yura, Takashi
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
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Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC91917/
https://ncbi.nlm.nih.gov/pubmed/10698746
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