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Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli

To examine the effects of overexpression of trigger factor (TF) on recombinant proteins produced in Escherichia coli, we constructed plasmids that permitted controlled expression of TF alone or together with the GroEL-GroES chaperones. The following three proteins that are prone to aggregation were...

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Main Authors: Nishihara, Kazuyo, Kanemori, Masaaki, Yanagi, Hideki, Yura, Takashi
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2000
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC91917/
https://ncbi.nlm.nih.gov/pubmed/10698746
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