Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli

To examine the effects of overexpression of trigger factor (TF) on recombinant proteins produced in Escherichia coli, we constructed plasmids that permitted controlled expression of TF alone or together with the GroEL-GroES chaperones. The following three proteins that are prone to aggregation were...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Nishihara, Kazuyo, Kanemori, Masaaki, Yanagi, Hideki, Yura, Takashi
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2000
Sujets:	Enzymology and Protein Engineering
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91917/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10698746/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.3.884-889.2000
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