Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli

To examine the effects of overexpression of trigger factor (TF) on recombinant proteins produced in Escherichia coli, we constructed plasmids that permitted controlled expression of TF alone or together with the GroEL-GroES chaperones. The following three proteins that are prone to aggregation were...

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Bibliographic Details
Main Authors: Nishihara, Kazuyo, Kanemori, Masaaki, Yanagi, Hideki, Yura, Takashi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC91917/
https://ncbi.nlm.nih.gov/pubmed/10698746
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