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Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium Shewanella Strain Ac10: Gene Cloning and Enzyme Purification and Characterization

The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of...

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Bibliographische Detailangaben
Hauptverfasser: Kulakova, Ljudmila, Galkin, Andrey, Kurihara, Tatsuo, Yoshimura, Tohru, Esaki, Nobuyoshi
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91069/
https://ncbi.nlm.nih.gov/pubmed/9925590
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