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Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium Shewanella Strain Ac10: Gene Cloning and Enzyme Purification and Characterization

The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of...

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Main Authors: Kulakova, Ljudmila, Galkin, Andrey, Kurihara, Tatsuo, Yoshimura, Tohru, Esaki, Nobuyoshi
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1999
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC91069/
https://ncbi.nlm.nih.gov/pubmed/9925590
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