Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium Shewanella Strain Ac10: Gene Cloning and Enzyme Purification and Characterization

The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Kulakova, Ljudmila, Galkin, Andrey, Kurihara, Tatsuo, Yoshimura, Tohru, Esaki, Nobuyoshi
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1999
Materias:	Enzymology and Protein Engineering
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91069/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9925590/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.2.611-617.1999
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