Cold-Adapted Alanine Dehydrogenases from Two Antarctic Bacterial Strains: Gene Cloning, Protein Characterization, and Comparison with Mesophilic and Thermophilic Counterparts

The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, She...

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Autores principales: Galkin, Andrey, Kulakova, Ljudmila, Ashida, Hiroyuki, Sawa, Yoshihiro, Esaki, Nobuyoshi
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 1999
Materias:
Enzymology and Protein Engineering
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC99735/
https://ncbi.nlm.nih.gov/pubmed/10473410
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