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Cold-Adapted Alanine Dehydrogenases from Two Antarctic Bacterial Strains: Gene Cloning, Protein Characterization, and Comparison with Mesophilic and Thermophilic Counterparts

The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, She...

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Udgivet i:Appl Environ Microbiol
Principais autores: Galkin, Andrey, Kulakova, Ljudmila, Ashida, Hiroyuki, Sawa, Yoshihiro, Esaki, Nobuyoshi
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1999
Fag:
Enzymology and Protein Engineering
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC99735/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10473410/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.9.4014-4020.1999
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