Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium Shewanella Strain Ac10: Gene Cloning and Enzyme Purification and Characterization

The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Kulakova, Ljudmila, Galkin, Andrey, Kurihara, Tatsuo, Yoshimura, Tohru, Esaki, Nobuyoshi
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Enzymology and Protein Engineering
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91069/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9925590/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.2.611-617.1999
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