DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening

Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA ele...

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Détails bibliographiques
Auteurs principaux: Sereno, Denis, Roy, Gaétan, Lemesre, Jean Loup, Papadopoulou, Barbara, Ouellette, Marc
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2001
Sujets:
Susceptibility
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC90440/
https://ncbi.nlm.nih.gov/pubmed/11257031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1168-1173.2001
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