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DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening
Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA ele...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC90440/ https://ncbi.nlm.nih.gov/pubmed/11257031 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1168-1173.2001 |
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