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DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening

Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA ele...

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Detalhes bibliográficos
Main Authors: Sereno, Denis, Roy, Gaétan, Lemesre, Jean Loup, Papadopoulou, Barbara, Ouellette, Marc
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC90440/
https://ncbi.nlm.nih.gov/pubmed/11257031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1168-1173.2001
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