DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening

Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA ele...

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Bibliographic Details
Main Authors: Sereno, Denis, Roy, Gaétan, Lemesre, Jean Loup, Papadopoulou, Barbara, Ouellette, Marc
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Susceptibility
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC90440/
https://ncbi.nlm.nih.gov/pubmed/11257031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1168-1173.2001
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