DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening

Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA ele...

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Autores principales: Sereno, Denis, Roy, Gaétan, Lemesre, Jean Loup, Papadopoulou, Barbara, Ouellette, Marc
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2001
Materias:
Susceptibility
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC90440/
https://ncbi.nlm.nih.gov/pubmed/11257031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1168-1173.2001
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