Peptide Deformylase as an Antibacterial Drug Target: Assays for Detection of Its Inhibition in Escherichia coli Cell Homogenates and Intact Cells

An assay was developed to determine the activity of peptide deformylase (PDF) inhibitors under conditions as close as possible to the physiological situation. The assay principle is the detection of N-terminal [(35)S]methionine labeling of a protein that contains no internal methionine. If PDF is ac...

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Detalhes bibliográficos

Publicado no:	Antimicrob Agents Chemother
Principais autores:	Apfel, Christian M., Evers, Stefan, Hubschwerlen, Christian, Pirson, Wolfgang, Page, Malcolm G. P., Keck, Wolfgang
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Mechanisms of Action: Physiological Effects
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90424/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11257015/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.45.4.1053-1057.2001
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