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Coupling Exonuclease Digestion with Selective Chemical Labeling forBase-resolution Mapping of 5-Hydroxymethylcytosine in Genomic DNA

This protocol is designed to obtain base-resolution information on the level of 5-hydroxymethylcytosine (5hmC) in CpGs without the need for bisulfite modification. It relies on (i) the capture of hydroxymethylated sequences by a procedure known as ‘selective chemical labeling’ (see Szulwach et al.,...

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Dettagli Bibliografici
Pubblicato in:Bio Protoc
Autori principali: Sérandour, Aurélien A., Avner, Stéphane, Salbert, Gilles
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-Protocol 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203863/
https://ncbi.nlm.nih.gov/pubmed/34179274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2747
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