Single-CpG resolution mapping of 5-hydroxymethylcytosine by chemical labeling and exonuclease digestion identifies evolutionarily unconserved CpGs as TET targets

Conventional techniques for single-base resolution mapping of epigenetic modifications of DNA such as 5-hydroxymethylcytosine (5hmC) rely on the sequencing of bisulfite-modified DNA. Here we present an alternative approach called SCL-exo which combines selective chemical labeling (SCL) of 5hmC in ge...

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Udgivet i:Genome Biol
Main Authors: Sérandour, Aurélien A., Avner, Stéphane, Mahé, Elise A., Madigou, Thierry, Guibert, Sylvain, Weber, Michaël, Salbert, Gilles
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4810514/
https://ncbi.nlm.nih.gov/pubmed/27025842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-0919-y
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