Characterizing Watson-Crick versus Hoogsteen base-pairing in a DNA-protein complex using NMR and site-specifically (13)C/(15)N labeled DNA

A(syn)-T and G(syn)-C(+) Hoogsteen base pairs in protein-bound DNA duplexes can be difficult to resolve by X-ray crystallography due to ambiguous electron density and by NMR spectroscopy due to poor chemical shift dispersion and size limitations with solution-state NMR spectroscopy. Here we describe...

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Veröffentlicht in:Biochemistry
Hauptverfasser: Zhou, Huiqing, Sathyamoorthy, Bharathwaj, Stelling, Allison, Xu, Yu, Xue, Yi, Pigli, Ying Zhang, Case, David A., Rice, Phoebe A., Al-Hashimi, Hashim M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8091065/
https://ncbi.nlm.nih.gov/pubmed/30950607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.9b00027
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