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A High-Throughput Assay to Identify Allosteric Inhibitors of the PLC-γ Isozymes Operating at Membranes

The two phospholipase C-γ (PLC-γ) isozymes are major signaling hubs and emerging therapeutic targets for various diseases, yet there are no selective inhibitors for these enzymes. We have developed a high-throughput, liposome-based assay that features XY-69, a fluorogenic, membrane-associated report...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Biochemistry
Hauptverfasser: Huang, Weigang, Carr, Adam J., Hajicek, Nicole, Sokolovski, Miri, Siraliev-Perez, Edhriz, Hardy, P. Brian, Pearce, Kenneth H., Sondek, John, Zhang, Qisheng
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8081235/
https://ncbi.nlm.nih.gov/pubmed/33028071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.0c00511
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