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Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts
Genetic manipulation of neural precursor cells is an important tool to study mechanisms underlying proliferation, fate specification, and neuron formation. The CRISPR/Cas9 system enables efficient genome editing but requires the clonal expansion of cells containing the desired mutation. Here, we des...
Tallennettuna:
| Julkaisussa: | STAR Protoc |
|---|---|
| Päätekijät: | , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Elsevier
2021
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8080521/ https://ncbi.nlm.nih.gov/pubmed/33948565 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100472 |
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