Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9

CRISPR/Cas9 technology enables the rapid generation of loss‐of‐function mutations in a targeted gene in mammalian cells. A single cell harboring those mutations can be used to establish a new cell line, thereby creating a CRISPR‐induced knockout clone. These clonal cell lines serve as crucial tools...

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Bibliografiske detaljer
Udgivet i:Curr Protoc Mol Biol
Main Authors: Giuliano, Christopher J., Lin, Ann, Girish, Vishruth, Sheltzer, Jason M.
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2019
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Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6741428/
https://ncbi.nlm.nih.gov/pubmed/31503414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.100
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