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Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9
Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made...
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| Pubblicato in: | STAR Protoc |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7566848/ https://ncbi.nlm.nih.gov/pubmed/33073252 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100052 |
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