Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology

Recent advances in genome engineering based on the CRISPR/Cas9 technology have revolutionized our ability to manipulate genomic DNA. Its use in human pluripotent stem cells (hPSCs) has allowed a wide range of mutant cell lines to be obtained at an unprecedented rate. The combination of these two gro...

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Main Authors: Joffrey Mianné, Chloé Bourguignon, Chloé Nguyen Van, Mathieu Fieldès, Amel Nasri, Said Assou, John De Vos
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2020-05-01
Colecção:Cells
Assuntos:
hPSC
CRISPR
screening
stem cells
gene editing
gene targeting
Acesso em linha:https://www.mdpi.com/2073-4409/9/5/1312
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