Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology

Recent advances in genome engineering based on the CRISPR/Cas9 technology have revolutionized our ability to manipulate genomic DNA. Its use in human pluripotent stem cells (hPSCs) has allowed a wide range of mutant cell lines to be obtained at an unprecedented rate. The combination of these two gro...

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Detalhes bibliográficos
Publicado no:Cells
Main Authors: Mianné, Joffrey, Bourguignon, Chloé, Nguyen Van, Chloé, Fieldès, Mathieu, Nasri, Amel, Assou, Said, De Vos, John
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Review
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7290981/
https://ncbi.nlm.nih.gov/pubmed/32466123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9051312
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