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An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system
Although the efficiency and versatility of CRISPR‐Cas9 system has been greatly improved over conventional genome editing methods such as zinc finger or TALEN, it is still time‐consuming and labor‐intensive for screening knockout/knock‐in cell clones due to differences of the targeted location or eff...
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| Publicado no: | Eng Life Sci |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7708952/ https://ncbi.nlm.nih.gov/pubmed/33304232 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.202000032 |
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