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An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system

Although the efficiency and versatility of CRISPR‐Cas9 system has been greatly improved over conventional genome editing methods such as zinc finger or TALEN, it is still time‐consuming and labor‐intensive for screening knockout/knock‐in cell clones due to differences of the targeted location or eff...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Eng Life Sci
Main Authors: Lu, Xibin, Guo, Yuhan, Gu, Shu, Tan, Deng, Cheng, Baoyun, Li, Zhoufang, Huang, Wei
Format: Artigo
Jezik:Inglês
Izdano: John Wiley and Sons Inc. 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708952/
https://ncbi.nlm.nih.gov/pubmed/33304232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.202000032
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