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Efficient and specific generation of knockout mice using Campylobacter jejuni CRISPR/Cas9 system
The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) system is now widely utilized to generate genome engineered mice; however, some studies raised issues related to off-target mutations with this system. Herein, we utilized the Campylobacter jejuni Cas9 (CjCas9) system to generate knockout mice. We desi...
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| 發表在: | Biochem Biophys Rep |
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| Main Authors: | , , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2020
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7118303/ https://ncbi.nlm.nih.gov/pubmed/32258440 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2020.100752 |
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