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Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency
The CRISPR/Cas9 system is widely applied in genome engineering due to its simplicity and versatility. Although this has revolutionized genome-editing technology, knockin animal generation via homology directed repair (HDR) is not as efficient as nonhomologous end-joining DNA-repair-dependent knockou...
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| Publicado no: | Exp Mol Med |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5938013/ https://ncbi.nlm.nih.gov/pubmed/29622782 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s12276-018-0037-x |
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