Amplification-free RNA detection with CRISPR–Cas13

CRISPR-based nucleic-acid detection is an emerging technology for molecular diagnostics. However, these methods generally require several hours and could cause amplification errors, due to the pre-amplification of target nucleic acids to enhance the detection sensitivity. Here, we developed a platfo...

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Bibliographic Details
Published in:Commun Biol
Main Authors: Shinoda, Hajime, Taguchi, Yuya, Nakagawa, Ryoya, Makino, Asami, Okazaki, Sae, Nakano, Masahiro, Muramoto, Yukiko, Takahashi, Chiharu, Takahashi, Ikuko, Ando, Jun, Noda, Takeshi, Nureki, Osamu, Nishimasu, Hiroshi, Watanabe, Rikiya
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2021
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8055673/
https://ncbi.nlm.nih.gov/pubmed/33875803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-021-02001-8
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