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CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium

CRISPR/Cas9 has emerged in various organisms as a powerful technology for targeted gene knockout; however, no reports of editing the Dictyostelium genome efficiently using this system are available. We describe here the application of CRISPR/Cas9-mediated gene modification in Dictyostelium. The endo...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Sekine, Ryoya, Kawata, Takefumi, Muramoto, Tetsuya
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5981456/
https://ncbi.nlm.nih.gov/pubmed/29855514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-26756-z
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