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Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase
The CRISPR/Cas9 system enables targeted genome modifications across a range of eukaryotes. Although we have reported that transient introduction of all-in-one vectors that express both Cas9 and sgRNAs can efficiently induce multiple gene knockouts in Dictyostelium discoideum, concerns remain about o...
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| Publicado no: | PLoS One |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6797129/ https://ncbi.nlm.nih.gov/pubmed/31622451 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0224128 |
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