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Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases

[Image: see text] Programmable CRISPR-Cas systems have augmented our ability to produce precise genome manipulations. Here we demonstrate and characterize the ability of CRISPR-Cas derived nickases to direct targeted recombination of both small and large genomic regions flanked by repetitive element...

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Bibliografske podrobnosti
izdano v:ACS Synth Biol
Main Authors: Standage-Beier, Kylie, Zhang, Qi, Wang, Xiao
Format: Artigo
Jezik:Inglês
Izdano: American Chemical Society 2015
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4655420/
https://ncbi.nlm.nih.gov/pubmed/26451892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.5b00132
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