Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy

The CRISPR-associated endonuclease Cas9 binds to a guide RNA and cleaves double-stranded DNA with a sequence complementary to the RNA guide. The Cas9–RNA system has been harnessed for numerous applications, such as genome editing. Here we use high-speed atomic force microscopy (HS-AFM) to visualize...

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Библиографические подробности
Опубликовано в: :Nat Commun
Главные авторы: Shibata, Mikihiro, Nishimasu, Hiroshi, Kodera, Noriyuki, Hirano, Seiichi, Ando, Toshio, Uchihashi, Takayuki, Nureki, Osamu
Формат: Artigo
Язык:Inglês
Опубликовано: Nature Publishing Group UK 2017
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5681550/
https://ncbi.nlm.nih.gov/pubmed/29127285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01466-8
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