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Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids
The S9.6 antibody is broadly used to detect RNA:DNA hybrids but has significant affinity for double-stranded RNA. The impact of this off-target RNA binding activity has not been thoroughly investigated, especially in the context of immunofluorescence microscopy. We report that S9.6 immunofluorescenc...
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| Publicado en: | J Cell Biol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Rockefeller University Press
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8040515/ https://ncbi.nlm.nih.gov/pubmed/33830170 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.202004079 |
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