The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast

R-loops, which result from the formation of stable DNA:RNA hybrids, can both threaten genome integrity and act as physiological regulators of gene expression and chromatin patterning. To characterize R-loops in fission yeast, we used the S9.6 antibody-based DRIPc-seq method to sequence the RNA stran...

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發表在:J Mol Biol
Main Authors: Hartono, Stella R., Malapert, Amélie, Legros, Pénélope, Bernard, Pascal, Chédin, Frédéric, Vanoosthuyse, Vincent
格式: Artigo
語言:Inglês
出版: 2017
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Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5987549/
https://ncbi.nlm.nih.gov/pubmed/29289567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2017.12.016
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