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A Crowding Barrier to Protein Inhibition in Colloidal Aggregates

Small molecule colloidal aggregates adsorb and partially denature proteins, inhibiting them artifactually. Oddly, this inhibition is typically time-dependent. Two mechanisms might explain this: low concentrations of the colloid and enzyme might mean low encounter rates, or colloid-based protein dena...

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Detalhes bibliográficos
Publicado no:J Med Chem
Main Authors: Lak, Parnian, O’Donnell, Henry, Du, Xuewen, Jacobson, Matthew P., Shoichet, Brian K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8035302/
https://ncbi.nlm.nih.gov/pubmed/33761256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jmedchem.0c02253
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