Accelerated RNA detection using tandem CRISPR nucleases

Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis of human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided recognition of RNA that triggers cleavage and release of a fluorescent reporter m...

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Publicado no:medRxiv
Main Authors: Liu, Tina Y., Knott, Gavin J., Smock, Dylan C. J., Desmarais, John J., Son, Sungmin, Bhuiya, Abdul, Jakhanwal, Shrutee, Prywes, Noam, Agrawal, Shreeya, de León Derby, María Díaz, Switz, Neil A., Armstrong, Maxim, Harris, Andrew R., Charles, Emeric J., Thornton, Brittney W., Fozouni, Parinaz, Shu, Jeffrey, Stephens, Stephanie I., Kumar, G. Renuka, Zhao, Chunyu, Mok, Amanda, Iavarone, Anthony T., Escajeda, Arturo M., McIntosh, Roger, Kim, Shin E., Dugan, Eli J., Pollard, Katherine S., Tan, Ming X., Ott, Melanie, Fletcher, Daniel A., Lareau, Liana F., Hsu, Patrick D., Savage, David F., Doudna, Jennifer A.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8010768/
https://ncbi.nlm.nih.gov/pubmed/33791736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/2021.03.19.21253328
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