Rapid, point-of-care molecular diagnostics with Cas13

Rapid nucleic acid testing is a critical component of a robust infrastructure for increased disease surveillance. Here, we report a microfluidic platform for point-of-care, CRISPR-based molecular diagnostics. We first developed a nucleic acid test which pairs distinct mechanisms of DNA and RNA ampli...

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Publié dans:medRxiv
Auteurs principaux: Agrawal, Shreeya, Fanton, Alison, Chandrasekaran, Sita S., Charrez, Bérénice, Escajeda, Arturo M., Son, Sungmin, Mcintosh, Roger, Bhuiya, Abdul, de León Derby, María Díaz, Switz, Neil A., Armstrong, Maxim, Harris, Andrew R., Prywes, Noam, Lukarska, Maria, Biering, Scott B., Smock, Dylan C. J., Mok, Amanda, Knott, Gavin J., Dang, Qi, Van Dis, Erik, Dugan, Eli, Kim, Shin, Liu, Tina Y., Harris, Eva, Stanley, Sarah A., Lareau, Liana F., Tan, Ming X., Fletcher, Daniel A., Doudna, Jennifer A., Savage, David F., Hsu, Patrick D.
Format: Artigo
Langue:Inglês
Publié: Cold Spring Harbor Laboratory 2021
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7755151/
https://ncbi.nlm.nih.gov/pubmed/33354689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/2020.12.14.20247874
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