An RNA in situ hybridization protocol optimized for monocot tissue

RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probe...

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Detaylı Bibliyografya
Yayımlandı:STAR Protoc
Asıl Yazarlar: Zöllner, Nora R., Bezrutczyk, Margaret, Laureyns, Reinout, Nelissen, Hilde, Simon, Rüdiger, Frommer, Wolf B.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2021
Konular:
Protocol
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7995656/
https://ncbi.nlm.nih.gov/pubmed/33796873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100398
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