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Protocol for Single-Molecule Fluorescence In Situ Hybridization for Intact Pancreatic Tissue
We describe an optimized smFISH protocol for the intact pancreas. The protocol is adapted from Lyubimova et al. (2013), a generic tissue smFISH protocol that works for most tissues but not the pancreas. The main changes implemented include increasing the period of mRNA denaturation from 5 min to at...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580104/ https://ncbi.nlm.nih.gov/pubmed/33111069 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2019.100007 |
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