dotdotdot: an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues

Multiplex single-molecule fluorescent in situ hybridization (smFISH) is a powerful method for validating RNA sequencing and emerging spatial transcriptomic data, but quantification remains a computational challenge. We present a framework for generating and analyzing smFISH data in complex tissues w...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Maynard, Kristen R, Tippani, Madhavi, Takahashi, Yoichiro, Phan, BaDoi N, Hyde, Thomas M, Jaffe, Andrew E, Martinowich, Keri
Format: Artigo
Language:Inglês
Published: Oxford University Press 2020
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7293004/
https://ncbi.nlm.nih.gov/pubmed/32383753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa312
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