Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Single-molecule fluorescence in situ hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target specificity...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Li, Xinbei, Eadara, Sreenivas, Jeon, Sangmin, Liu, Yan, Muwanga, Gabriella, Qu, Lintao, Caterina, Michael J., Meffert, Mollie K.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8185307/
https://ncbi.nlm.nih.gov/pubmed/34142098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100555
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