Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Single-molecule fluorescence in situ hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target specificity...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:STAR Protoc
Päätekijät: Li, Xinbei, Eadara, Sreenivas, Jeon, Sangmin, Liu, Yan, Muwanga, Gabriella, Qu, Lintao, Caterina, Michael J., Meffert, Mollie K.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2021
Aiheet:
Protocol
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC8185307/
https://ncbi.nlm.nih.gov/pubmed/34142098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100555
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