An RNA in situ hybridization protocol optimized for monocot tissue

RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probe...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:STAR Protoc
Päätekijät: Zöllner, Nora R., Bezrutczyk, Margaret, Laureyns, Reinout, Nelissen, Hilde, Simon, Rüdiger, Frommer, Wolf B.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2021
Aiheet:
Protocol
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7995656/
https://ncbi.nlm.nih.gov/pubmed/33796873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100398
Tagit: Lisää tagi
Ei tageja, Lisää ensimmäinen tagi!

Samankaltaisia teoksia