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An RNA in situ hybridization protocol optimized for monocot tissue
RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probe...
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| Pubblicato in: | STAR Protoc |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7995656/ https://ncbi.nlm.nih.gov/pubmed/33796873 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100398 |
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