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An optimized protocol for high-throughput in situ hybridization of zebra finch brain
In situ hybridization (ISH) is a sensitive technique for documenting the tissue distribution of mRNAs. Advances in non-radioactive methods based on chromogenic detection of digoxigenin (DIG)-labeled probes have increased spatial resolution compared to emulsion autoradiography, and when paired with h...
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| Publicado no: | Cold Spring Harb Protoc |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4589143/ https://ncbi.nlm.nih.gov/pubmed/25342071 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot084582 |
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