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An optimized protocol for high-throughput in situ hybridization of zebra finch brain

In situ hybridization (ISH) is a sensitive technique for documenting the tissue distribution of mRNAs. Advances in non-radioactive methods based on chromogenic detection of digoxigenin (DIG)-labeled probes have increased spatial resolution compared to emulsion autoradiography, and when paired with h...

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Detalles Bibliográficos
Publicado en:Cold Spring Harb Protoc
Main Authors: Carleton, J., Lovell, P.V., McHugh, A., Marzulla, T., Horback, K., Mello, C.V.
Formato: Artigo
Idioma:Inglês
Publicado: 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4589143/
https://ncbi.nlm.nih.gov/pubmed/25342071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot084582
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