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An optimized protocol for high-throughput in situ hybridization of zebra finch brain

In situ hybridization (ISH) is a sensitive technique for documenting the tissue distribution of mRNAs. Advances in non-radioactive methods based on chromogenic detection of digoxigenin (DIG)-labeled probes have increased spatial resolution compared to emulsion autoradiography, and when paired with h...

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Dettagli Bibliografici
Pubblicato in:Cold Spring Harb Protoc
Autori principali: Carleton, J., Lovell, P.V., McHugh, A., Marzulla, T., Horback, K., Mello, C.V.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4589143/
https://ncbi.nlm.nih.gov/pubmed/25342071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot084582
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