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Removal of Affinity Tags with TEV Protease
Although affinity tags are highly effective tools for the expression and purification of recombinant proteins, they generally need to be removed prior to structural and functional studies. This chapter describes a simple method for overproducing a soluble form of a stable variant of tobacco etch vir...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7974378/ https://ncbi.nlm.nih.gov/pubmed/28470608 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6887-9_14 |
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