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Expression and Purification of Recombinant Proteins in Escherichia coli with a His(6) or Dual His(6)-MBP Tag
Rapid advances in bioengineering and biotechnology over the past three decades have greatly facilitated the production of recombinant proteins in Escherichia coli. Affinity-based methods that employ protein or peptide based tags for protein purification have been instrumental in this progress. Yet i...
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| Publié dans: | Protein Crystallography |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7122414/ https://ncbi.nlm.nih.gov/pubmed/28573567 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7000-1_1 |
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