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Expression and Purification of Recombinant Proteins in Escherichia coli with a His(6) or Dual His(6)-MBP Tag

Rapid advances in bioengineering and biotechnology over the past three decades have greatly facilitated the production of recombinant proteins in Escherichia coli. Affinity-based methods that employ protein or peptide based tags for protein purification have been instrumental in this progress. Yet i...

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Detalhes bibliográficos
Publicado no:Protein Crystallography
Main Authors: Raran-Kurussi, Sreejith, Waugh, David S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7122414/
https://ncbi.nlm.nih.gov/pubmed/28573567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7000-1_1
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