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Gateway vectors for the production of combinatorially-tagged His(6)-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli
Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2253240/ https://ncbi.nlm.nih.gov/pubmed/16322578 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051718605 |
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